716 functional behavior of a positive edge triggered d flip flop
Tài liệu Báo cáo Y học: Functional analysis of a small heat shock/a-crystallin protein from Artemia franciscana docx
Ngày tải lên :
22/02/2014, 04:20
... GCGCGGATCCACCATGGCACTTAACCCATG CGCGCCTCGAGTTAAGCTGCACCTCCTGATCT GCGCGGATCCACCATGCCCTTCCGGAGAAGA CGCGCCTCGAGTTAAGCTGCACCTCCTGATCT GCGCGGATCCACCATGTCCTTGAGGGACACA CGCGCCTCGAGTTAAGCTGCACCTCCTGATCT GCGCGGATCCACCATGGCACTTAACCCATG ... GCGCGGATCCACCATGGCACTTAACCCATG CGCGCCTCGAGTTAACGTTCTGTTGGTGAGCT GCGCGGATCCACCATGGCACTTAACCCATG CGCGCCTCGAGTTATGGAGTTGAACTAGCTGT GCGCGGATCCACCATGTCCTTGAGGGACACA CGCGCCTCGAGTTAACGTTCTGTTGGTGAGCT Length (bp/amino acids) ... Additionally, 400 lL of p26 purified from Artemia cysts [57], and molecular mass markers of 29 kDa (carbonic anhydrase), 66 kDa (bovine serum albumin), 150 kDa (alcohol dehydrogenase), 200 kDa...
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Báo cáo khoa học: Functional analysis of a murine monoclonal antibody against the repetitive region of the fibronectin-binding adhesins fibronectin-binding protein A and fibronectin-binding protein B from Staphylococcus aureus pot
Ngày tải lên :
06/03/2014, 22:21
... SH1000 (D, E) After several washings, lg of rabbit polyclonal antibody against S aureus was added Bound antibody was detected by incubation with secondary antibody [HRP-conjugated goat anti-(rabbit ... incubated with 10 lg of Fn and, after washing, bound Fn was visualized by incubation with rabbit polyclonal antibody against Fn followed by addition of peroxidase-conjugated goat anti-(rabbit ... filter) Data were recorded and analyzed with flowmax software from Partec Statistical analysis of ELISA experiments Each experiment was repeated at least twice, with duplicate or triplicate measurements...
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Báo cáo khoa học: Molecular and functional characterization of a novel splice variant of ANKHD1 that lacks the KH domain and its role in cell survival and apoptosis docx
Ngày tải lên :
07/03/2014, 21:20
... (Forward) TAAGCTACTACGTAAAGAATATATC (Reverse) GATAAGGTACCTGCACTGACACGGATGAAAGC (Forward) CATATATTCTTTACGTAGTAGCTTA (Reverse) FEBS Journal 272 (2005) 4091–4102 ª 2005 FEBS 4099 Molecular characterization ... 627 and 435 amino acids and with calculated peptide masses of 69 and 49 kDa, respectively Additional domain mapping revealed that the predicted structure of VBARP does not contain signal peptide(s) ... Molecular characterization of ANKHD1 splice variant studies blast searches of VBARP revealed that this protein has homology to human ankyrin repeat and KH domain containing 1(ANKHD1) variants, and...
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Báo cáo khoa học: Identification and functional expression of a second human b-galactoside a2,6-sialyltransferase, ST6Gal II docx
Ngày tải lên :
08/03/2014, 08:20
... generated by PCR from HepG2 cDNA library (C Baisez and A Harduin-Lepers, unpublished data), as the template and two specific primers For 6I 5¢-CGATGAATTC GTTAACGCTCATCACCATCACCATCACGGGAAA TTGGCCATGGGGT-3¢ ... radioactive products were detected and quantified by radio-imaging using a Personal Molecular Imager FX (Bio-Rad, France) Quantification was performed within the linear range of standard radioactivity ... for h Digested materials were then analysed by SDS/PAGE and the radioactive products were analysed by radio-imaging Results Identification and isolation of human ST6Gal II cDNA Similarity searches...
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Báo cáo khoa học: Identification and functional characterization of a novel barnacle cement protein pptx
Ngày tải lên :
16/03/2014, 05:20
... TSVSAGDGAFGNLAAALTLVEDTEDGLGVKTKNGGKGFSEGTAAISQTAGANGGATVKKA VSASAANGFFKNLGKATTEVKTTKDGTKVKTKTAGKGKTGGTATTIQIADANGGVSEKSL AAAAAGNGVFKNLVTALTNISTTDDITKVQTQTIGSGGTGGAATILQLADANGGAALKEV Mrcp19k Bacp19k Bicp19k 130 140 150 160 170 KLDLLTDGEDLFDTKKVEKGTVTSSSSHQGSGAGDSIFEILNEAESKIKKSGD ... supernatant was recovered as GSF2 Both fractions were dialyzed against 1% acetic acid at °C, before being evaporated and stored at ) 20 °C until needed GSF1 and GSF2 were separated by SDS ⁄ PAGE (a ... Mountain View, CA, USA), and poly (A) + RNA was isolated using Oligo(dT)-Latex Super (Takara Shuzo Co.) cDNA was prepared from mRNA with a Zap-cDNA synthesis kit (Stratagene, La Jolla, CA, USA) according...
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Báo cáo khoa học: Functional importance of a conserved sequence motif in FhaC, a prototypic member of the TpsB/Omp85 superfamily ppt
Ngày tải lên :
23/03/2014, 03:20
... Data Bank database under the accession number 3NJT Overlay assay Channel analysis Fha30 is a 30-kDa, secreted FHA truncate encompassing the TPS domain and first three repeats It was used as bait ... bait and FhaCmyc and its variants as prey Fha30 was produced and purified as described previously [15] Identical amounts of Fha30 (5 lg) were loaded onto several lanes of SDS ⁄ PAGE gels and, following ... arguing against a major conformational change of the loop Structural data are available in the Protein Data Bank under the accession numbers 2QDZ (FhaCWT) and 3NJT (FhaCR45 0A) voltage sweep directions...
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Báo cáo khoa học: Functional dissection of a small anaerobically induced bZIP transcription factor from tomato pdf
Ngày tải lên :
23/03/2014, 13:20
... TACTCGAGATGTCACCTTTAAGGCAGAG-3¢ and 5¢-ATATCCATGGAAAATTTAAACAATCCTGATG-3¢ Abz1(1–44): 5¢-TATACTCGAGATGTCACCTTTAAG GCAGAG-3¢ and 5¢-ATATCCATGGGCTTCTTCATC CTCGATCGC-3¢ Abz1(1–24): 5¢-TATACTCGAGAT ... 5¢-TATACTCGAGAT GTCACCTTTAAGGCAGAG-3¢ and 5¢-ATATCCATG GTCTCATCCATTCCTGCATAC-3¢ Abz1(45–138): 5¢TATACTCGAGATGCAGAAGCTTCTGCAAGATTT GAC-3¢ and 5¢-ATATCCATGGAAAATTTAAACAA TCCTGATG-3¢ The XhoI and NcoI sites ... TCCATGTCACCTTTAAGGCAGAG-3¢ and 5¢-ATAT CCCGGGTTAAAATTTAAACAATCCTG-3¢ N-terminal deletion, ABZ1(47–138): 5¢-TATGGATCCATGC TTCTGCAAGATTTGACAGG-3¢ and 5¢-ATATCCC GGGTTAAAATTTAAACAATCCTG-3¢ C-terminal deletion, ABZ1(1–100):...
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Báo cáo Y học: Structural and functional characterization of a C-type lectin-like antifreeze protein from rainbow smelt (Osmerus mordax) potx
Ngày tải lên :
24/03/2014, 03:21
... N-glycosidase F added h later, and again after an overnight incubation A further U was added over a period of h followed by a second overnight incubation at 37 °C The reaction was considered complete ... red staining of smelt AFP Ruthenium red (RR) staining of deglycosylated (D) smelt AFP and untreated (U) smelt AFP was carried out as described in Materials and methods Blots shown are stained ... formed products that were kDa and kDa smaller than undigested smelt AFP The size differences between the digestion fragments of untreated and deglycosylated AFP 1222 J C Achenbach and K V Ewart...
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Báo cáo khoa học: Molecular cloning and functional expression of a gene encoding an antiarrhythmia peptide derived from the scorpion toxin pptx
Ngày tải lên :
31/03/2014, 09:20
... obtained was displayed an ORF of 258 bp encoding a polypeptide of 85 amino acids and termed BmKIM The 5¢ and 3¢ UTRs of BmKIM cDNA are 17 bp and 76 bp, respectively A single AATAAA polyadenylation ... (ventricular tachycardia), and VF (ventricular fibrillation) appeared were noted and recoded; the cumulative aconitine dosage to induce PVC, VT, VF was calculated Data are expressed as mean ± SEM Differences ... 47 6A sequencer was used for automated Edman degradation The phenylthiohydantoin derivatives of the amino acids were identified using an Applied Biosystems Model 12 0A PTH-Analyzer Circular dichroism...
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Báo cáo hóa học: " Dynamic behavior of a nonlinear rational difference equation and generalization" ppt
Ngày tải lên :
20/06/2014, 22:20
... article as: Shi et al.: Dynamic behavior of a nonlinear rational difference equation and generalization Advances in Difference Equations 2011 2011:36 Submit your manuscript to a journal and benefit ... Global Behavior of Nonlinear Difference Equations of Higher Order with Application Kiuwer Academic Publishers, Dordrecht (1993) Kulenovic, MR., Ladas, G: Dynamics of Second Order Rational Difference ... participated in the design and coordination All authors read and approved the final manuscript Competing interests The authors declare that they have no competing interests Received: 17 May 2011 Accepted:...
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Báo cáo hóa học: " Qualitative behavior of a rational difference equation y = py++y yy" pdf
Ngày tải lên :
21/06/2014, 03:20
... participated in the design and coordination All authors read and approved the final manuscript Competing interests The authors declare that they have no competing interests Received: 10 February ... Ladas G: Dynamics of Second Order Rational Difference Equations with Open Problems and Conjectures Chapman & Hall/CRC Press; 2001 Elabbasy EM, El-Metwally H, Elsayed EM: On the difference equation ... equilibrium point x = of ¯ Equation 15 is locally asymptotically stable with initial data x0 = 1, x1 = 1.2 Authors’ contributions Xiao Qian carried out the theoretical proof and drafted the manuscript Shi...
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Báo cáo hóa học: " Research Article Asymptotic Behavior of a Periodic Diffusion System" pot
Ngày tải lên :
21/06/2014, 07:20
... 1983 24 O Ladyzenskaja, V Solonnikov, and N Uraltseva, “Linear and quasilinear equations of parabolic type,” in Translations of Mathematical Monographs, vol 23, American Mathematical Society, ... Formation, Academic Press, London, UK, 1982 Yu M Romanovski˘, N V Stepanova, and D S Chernavski˘, Matematicheskaya Biofizika, Nauka, ı ı Moscow, Russia, 1984 J Bebernes and D Eberly, Mathematical ... “Global existence and blow-up for a nonlinear reaction-diffusion system,” Journal of Mathematical Analysis and Applications, vol 212, no 2, pp 481–492, 1997 Journal of Inequalities and Applications...
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Báo cáo hóa học: "ASYMPTOTIC BEHAVIOR OF A COMPETITIVE SYSTEM OF LINEAR FRACTIONAL DIFFERENCE EQUATIONS" docx
Ngày tải lên :
22/06/2014, 22:20
... ∞ A fixed point E is a global attractor on a set if E is an attractor and is a subset of the basin of attraction of E If F is differentiable at a fixed point E, and if the Jacobian JF (E) has ... formulate this statement as a conjecture In [12] we showed that in cases (5) and (6) an introduction of the positive parameters a and d changed the global behavior of system (1.2) while in case ... eigenvalue with modulus less than one and a second eigenvalue with modulus greater than one, E is said to be a saddle See [15] for additional definitions 4 Competitive system of rational difference...
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Báo cáo hóa học: "GLOBAL BEHAVIOR OF A HIGHER-ORDER RATIONAL DIFFERENCE EQUATION" doc
Ngày tải lên :
22/06/2014, 22:20
... 4707–4717 [10] G Papaschinopoulos and C J Schinas, Global asymptotic stability and oscillation of a family of difference equations, Journal of Mathematical Analysis and Applications 294 (2004), ... Mathematics, Guangxi College of Finance and Economics, Nanning, Guangxi 530004, China E-mail address: xhongjian@263.com Taixiang Sun: Department of Mathematics, College of Mathematics and Information ... for a fourth-order rational difference equation, Journal of Mathematical Analysis and Applications 312 (2005), no 2, 555–563 , Qualitative properties for a fourth-order rational difference equation,...
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Báo cáo hóa học: " Research Article Analysis of Transient and Steady-State Behavior of a Multichannel Filtered-x Partial-Error Affine Projection Algorithm" docx
Ngày tải lên :
22/06/2014, 22:20
... Steady-state behavior We are here interested in the estimation of the mean-square error (MSE) and the mean-square deviation (MSD) at steady state The adaptation rule of (15) provides different values ... this paper, we extend the analysis approach of [4] and study the transient and steady-state behavior of a filtered-x partial error a ne projection (FX-PE-AP) algorithm The paper shows that the ... million samples From Figures and 4, we see that the expressions in (33) and in (34) provide accurate estimates of the steady-state MSE and of the steady-state A Carini and G L Sicuranza Table 3:...
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BEHAVIOR OF THE POSITIVE SOLUTIONS OF FUZZY MAX-DIFFERENCE EQUATIONS G. STEFANIDOU AND G. pptx
Ngày tải lên :
23/06/2014, 00:20
... (i) of Lemma 5.1 for any a ∈ (0,1] − E there exists a wa ∈ {1,2,3} and an ∈ {0,1, } such that uwa ,a < A1 ,l ,a , A1 ,l ,a A1,r ,a A2 +1 ≤ uwa ,a A1,l ,a A1,r ,a ≤ A1 ,l ,a A2 (5.17) Hence, from statement ... exists an a ∈ (0,1] which satisfies one of the the following conditions: (i) A0 ,l ,a < A0 ,r ,a , A1 ,l ,a < A1 ,r ,a , (ii) A0 ,l ,a < A0 ,r ,a , A1 ,l ,a < A0 ,r ,a , (iii) A0 ,l ,a < A1 ,r ,a , A1 ,l ,a < A1 ,r ,a , ... 0,1, ,a ∈ (0,1], be the unique positive solution of the system of difference equations Al ,a Al ,a A , , , l ,a , Rn ,a Rn−1 ,a Rn−k ,a Ar ,a Ar ,a A Rn+1 ,a = max , , , r ,a Ln ,a Ln−1 ,a Ln−k ,a Ln+1 ,a = max...
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EXISTENCE OF A POSITIVE SOLUTION FOR A p-LAPLACIAN SEMIPOSITONE PROBLEM MAYA CHHETRI AND R. SHIVAJI docx
Ngày tải lên :
23/06/2014, 00:20
... USA E-mail address: maya@uncg.edu R Shivaji: Department of Mathematics and Statistics, Mississippi State University, Mississippi State, MS 39762, USA E-mail address: shivaji@math.msstate.edu ... search Notes in Mathematics, vol 404, Chapman & Hall/CRC, Florida, 1999 Z M Guo and J R L Webb, Large and small solutions of a class of quasilinear elliptic eigenvalue problems, J Differential ... quasilinear a a elliptic problems, Nonlinear Anal Ser A: Theory Methods 44 (2001), no 2, 189–204 P Dr´ bek, P Krejˇ ´, and P Tak´ c, Nonlinear Differential Equations, Chapman & Hall/CRC Rea cı a ...
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Báo cáo y học: "Structural and functional map of a bacterial nucleoid" doc
Ngày tải lên :
09/08/2014, 20:21
... early, mid, and late exponential and early and late stationary phases With these data, investigators should be able to obtain a dynamic picture of protein occupancy for NAPs along the different ... was supported during her PhD studies (Programa de Doctorado en Ciencias Biomédicas, Universidad Nacional Autónoma de México) by a fellowship from the Consejo Nacional de Ciencia y Tecnolog a ... Jiménez-Jacinto V, Peralta-Gil M, SantosZavaleta A, Peñaloza-Spinola MI, Contreras-Moreira B, Segura-Salazar J, Muñiz-Rascado L, Martínez-Flores I, Salgado H, Bonavides-Martínez C, Abreu-Goodger C, http://genomebiology.com/2009/10/12/247...
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báo cáo khoa học: " Isolation and functional characterization of a cDNA coding a hydroxycinnamoyltransferase involved in phenylpropanoid biosynthesis in Cynara cardunculus L" potx
Ngày tải lên :
12/08/2014, 05:20
... 5'-CCCGACGATCAGGATA-3' 5'-ACCGCCGGGATGAGTT-3' 5'-CCGCCTCCACGAACAA-3' 5'-TTCCGTTTCGTTTCTTCAA-3' 5'-TGGCCATAACCATTTTAGATAT-3' 5'-GGGTTTCATATGAAGATCGAGGTGAGAGAA-3' 5'-CGGGATCCTTAGATATCATATAGGAACTTGC-3' ... upstream and downstream of the 3'-hydroxylation step In addition, for both wild and Plant material and RNA extraction Leaves of globe artichoke, cultivated cardoon and wild cardoon were collected ... performed the HPLC's All authors read and approved the final manuscript Acknowledgements We thank Prof G Mauromicale and Dr R Mauro of the University of Catania for providing plant material We are particularly...
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